Microscopy in Microbiology
Biology ⇒ Microorganisms and Microbiology
Microscopy in Microbiology starts at 8 and continues till grade 12.
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See sample questions for grade 11
A student is using a microscope with a 10x ocular lens and a 100x oil immersion objective lens. What is the total magnification?
A student observes a bacterial cell under a microscope using a 10x ocular lens and a 40x objective lens. What is the total magnification?
Describe one advantage and one limitation of using electron microscopy in microbiology.
Describe the main advantage of using fluorescence microscopy in microbiology.
Describe the principle of dark-field microscopy.
Describe the purpose of staining in light microscopy.
Explain the main difference between a scanning electron microscope (SEM) and a transmission electron microscope (TEM).
Explain the main reason why staining is not required for phase-contrast microscopy.
Explain why electron microscopes cannot be used to observe living cells.
Explain why immersion oil is used with the 100x objective lens in light microscopy.
Explain why the use of a cover slip is important when preparing a wet mount for light microscopy.
A microbiologist is comparing the images of a bacterial cell obtained using phase-contrast microscopy and differential interference contrast (DIC) microscopy. What is one key difference in the appearance of the images produced by these two techniques?
Calculate the resolving power (d) of a light microscope using the following formula: d = λ / (2NA), where λ = 550 nm and the numerical aperture (NA) of the objective lens is 1.25. Give your answer in nanometers.
Describe the principle behind super-resolution microscopy and explain how it overcomes the diffraction limit of light.
Explain how confocal laser scanning microscopy improves the resolution and contrast of thick biological specimens compared to conventional fluorescence microscopy.
Explain why the use of monochromatic light can improve the resolution in light microscopy.
