Microscopy in Microbiology
Biology ⇒ Microorganisms and Microbiology
Microscopy in Microbiology starts at 8 and continues till grade 12.
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See sample questions for grade 12
A microbiologist observes a specimen using a 100x oil immersion objective and a 10x ocular lens. What is the total magnification?
A student observes a bacterial cell under a microscope using a 10x ocular lens and a 40x objective lens. What is the total magnification?
Describe one advantage and one limitation of using fluorescence microscopy in microbiology.
Describe the main difference between simple and compound microscopes.
Describe the principle of dark-field microscopy.
Explain the main difference between a scanning electron microscope (SEM) and a transmission electron microscope (TEM).
Explain why electron microscopes have a higher resolving power than light microscopes.
Explain why it is important to start focusing with the lowest power objective lens when using a compound microscope.
Explain why it is important to use the oil immersion lens when observing bacteria at high magnification.
Name one reason why staining is not always used when observing microorganisms under a microscope.
Name one type of microscopy that allows visualization of three-dimensional surface structures of microorganisms.
What is the function of immersion oil in light microscopy?
What is the main advantage of using a confocal laser scanning microscope in microbiology?
What is the main limitation of using a bright-field microscope for observing unstained bacteria?
What is the main purpose of staining specimens in light microscopy?
A microbiologist wants to observe the dynamic process of bacterial cell division in real time. Which type of microscopy would be most appropriate and why?
A researcher is studying the localization of a specific protein within a bacterial cell. Which advanced microscopy technique would be most suitable for this purpose, and why?
Describe how super-resolution microscopy techniques, such as STED or PALM, have advanced the study of microorganisms beyond the limits of conventional light microscopy.
Explain how the use of differential interference contrast (DIC) microscopy enhances the visualization of microbial structures compared to phase-contrast microscopy.
Explain the importance of numerical aperture (NA) in determining the resolving power of a microscope, and how increasing NA affects image quality.
